# Hodgkin-Huxley model in R

One of the great research papers of the 20th century celebrates its 60th anniversary in a few weeks time: *A quantitative description of membrane current and its application to conduction and excitation in nerve* by Alan Hodgkin and Andrew Huxley. Only shortly after Andrew Huxley died, 30th May 2012, aged 94.

In 1952 Hodgkin and Huxley published a series of papers, describing the basic processes underlying the nervous mechanisms of control and the communication between nerve cells, for which they received the Nobel prize in physiology and medicine, together with John Eccles in 1963.

Their research was based on electrophysiological experiments carried out in the late 1940s and early 1950 on a giant squid axon to understand how action potentials in neurons are initiated and propagated.The capacitive current across the cell membrane can be described as the sum of changes in the membrane voltage \(V_m\) and ion currents caused primarily by sodium (Na) and potassium (K) and other leakages (L), mainly chloride ions. The ion currents are defined by their conductances (\(g\), with the Na and K conductances being voltage depended), their equilibrium potentials (\(E\)) and how the channel gates open and close (\(m, n, h\)): \[ \begin{align} I_{ext} &= C_m \frac{dV_m}{dt} + I_{ion}\\ & = C_m \frac{dV_m}{dt} + g_{Na} m^3 h(V-E_{Na}) + g_K n^4 (V - E_K) + g_L (V - E_L) \end{align} \]

The standard Hodgkin-Huxley model expands into a set of four differential equations: \[ \begin{align} C \frac{dV}{dt} & = I - g_{Na} m^3 h(V-E_{Na}) - g_K n^4 (V - E_K) -g_L (V - E_L)\\ \frac{dm}{dt} & = a_m(V) (1 - m) - b_m(V)m\\ \frac{dh}{dt} & = a_h(V)(1 - h) - b_h(V)h\\ \frac{dn}{dt} & = a_n(V) (1 - n) - b_n(V)n\\ a_m(V) & = 0.1(V+40)/(1 - \exp(-(V+40)/10))\\ b_m(V) & = 4 \exp(-(V + 65)/18)\\ a_h(V) & = 0.07 \exp(-(V+65)/20)\\ b_h(V) & = 1/(1 + \exp(- (V + 35)/10))\\ a_n(V) & = 0.01 (V + 55)/(1 - \exp(-(V+55)/10))\\ b_n(V) & = 0.125 \exp(-(V + 65)/80) \end{align} \]

To model and simulate those differential equations in R I use the `simecol`

package. The package comes with a great vignette which gives a good introduction, and you may also find my recent LondonR talk helpful.

```
library(simecol)
## Hodkin-Huxley model
HH <- odeModel(
main = function(time, init, parms) {
with(as.list(c(init, parms)),{
am <- function(v) 0.1*(v+40)/(1-exp(-(v+40)/10))
bm <- function(v) 4*exp(-(v+65)/18)
ah <- function(v) 0.07*exp(-(v+65)/20)
bh <- function(v) 1/(1+exp(-(v+35)/10))
an <- function(v) 0.01*(v+55)/(1-exp(-(v+55)/10))
bn <- function(v) 0.125*exp(-(v+65)/80)
dv <- (I - gna*h*(v-Ena)*m^3-gk*(v-Ek)*n^4-gl*(v-El))/C
dm <- am(v)*(1-m)-bm(v)*m
dh <- ah(v)*(1-h)-bh(v)*h
dn <- an(v)*(1-n)-bn(v)*n
return(list(c(dv, dm, dh, dn)))
})
},
## Set parameters
parms = c(Ena=50, Ek=-77, El=-54.4, gna=120, gk=36, gl=0.3, C=1, I=0),
## Set integrations times
times = c(from=0, to=40, by = 0.25),
## Set initial state
init = c(v=-65, m=0.052, h=0.596, n=0.317),
solver = "lsoda"
)
```

Let’s run and plot the model:

```
HH <- sim(HH)
plot(HH)
```

From the initial state I observe a tiny stimulus which reverts quickly back to the resting state. So let’s increase the external stimulus in steps to observe its impact on the membrane voltage:

```
times(HH)["to"] <- 100
## Stimulus
I <- c(2, 5, 5.97, 5.975, 6.2, 6.5)
sims <- do.call("rbind",
lapply(I, function(i){
parms(HH)["I"] <- i
HH <- sim(HH)
cbind(I=paste("I =", i), out(HH))
}))
## Plot the various experiments with lattice
library(latticeExtra)
asTheEconomist(
xyplot(v ~ time | factor(I), data=sims, type="l",
as.table=TRUE,
main="Hodgkin-Huxely model with varying external stimulus"),
xlab="time (ms)", ylab="mV")
```

Now this is exciting, as I increase the external stimulus an action potential is generated. Increasing the stimulus further results in a constant firing of the neuron, or in other words, the model is going through a Hopf-bifurcation. I think it is absolutely remarkable what Hodgkin and Huxley achieved 60 years ago. They were able to demonstrate that numerical integration of their model could reproduce all the key biophysical properties of the action potential. And what seems so easy on a computer with R or other software, such as XPP/XPPAUT today, must have been a lot of work in the late 40s, early 50s of the 20th century.